1 AIT Asian Institute of Technology

Evaluation of prebiotics and encapsulation on the characteristics of probiotic bacteria

AuthorBussarin Kosin
Call NumberAIT Thesis no. BP-02-06
Subject(s)Microencapsulation--Evaluation

NoteA thesis submitted in partial fulfillment of the requirement for the degree of Master of Science, School of Environment, Resources and Development
PublisherAsian Institute of Technology
Series StatementThesis ; no. BP-02-06
AbstractA strain of Lactobacillus brevis, isolated from Thai traditional fermented vegetable was found to be a better probiotic strain than some other commercial strains due to its highest potential in having desired probiotic characteristics which are acid to lerance, bile tolerance and antagonistic effect against pathogenic bacteria. A modified method using calcium alginate for the microencapsulation of this probiotic bacteria introducing the incorporation of different prebiotics (fructooligosaccharide, oligofructose, high amylase corn resistant starch and maltooligosacchride) was used to develop a synbiotic system. Incorporation of high amylase corn resistant starch (a prebiotic) improved an efficiency in encapsulation of viable bacteria. The encapsulated bacteria, however, did not demonstrate a significant increase in survival when subjected to in vitro high acid and bi le salt conditions when compared with encapsulation with calcium alginate but it showed a significant increase in survival when compared with free cells: A preliminary study was carried out in order to monitor the effects of encapsulation with resistant starch on the survival of probiotic L. brevis in yogurt product for a shelf life of 4 weeks at 4°C. This study showed that the survival of encapsulated culture of L. brevis showed a decline in viable count of about 0.9 log over a period of 4 weeks while there was a decline of about 1.6 log in cultures which were incorporated as free cells in yogurt.
Year2002
Corresponding Series Added EntryAsian Institute of Technology. Thesis ; no. BP-02-06
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSBioprocess Technology (BP)
Chairperson(s)Rakshit, Sudip K.;
Examination Committee(s)Athapol Noomhorm;Jindal, Vinod K.;
Scholarship Donor(s)Royal Thai Government;
DegreeThesis (M. Sc.) - Asian Institute of Technology, 2002


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