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Evaluation of prebiotics and encapsulation on the characteristics of probiotic bacteria | |
Author | Bussarin Kosin |
Call Number | AIT Thesis no. BP-02-06 |
Subject(s) | Microencapsulation--Evaluation |
Note | A thesis submitted in partial fulfillment of the requirement for the degree of Master of Science, School of Environment, Resources and Development |
Publisher | Asian Institute of Technology |
Series Statement | Thesis ; no. BP-02-06 |
Abstract | A strain of Lactobacillus brevis, isolated from Thai traditional fermented vegetable was found to be a better probiotic strain than some other commercial strains due to its highest potential in having desired probiotic characteristics which are acid to lerance, bile tolerance and antagonistic effect against pathogenic bacteria. A modified method using calcium alginate for the microencapsulation of this probiotic bacteria introducing the incorporation of different prebiotics (fructooligosaccharide, oligofructose, high amylase corn resistant starch and maltooligosacchride) was used to develop a synbiotic system. Incorporation of high amylase corn resistant starch (a prebiotic) improved an efficiency in encapsulation of viable bacteria. The encapsulated bacteria, however, did not demonstrate a significant increase in survival when subjected to in vitro high acid and bi le salt conditions when compared with encapsulation with calcium alginate but it showed a significant increase in survival when compared with free cells: A preliminary study was carried out in order to monitor the effects of encapsulation with resistant starch on the survival of probiotic L. brevis in yogurt product for a shelf life of 4 weeks at 4°C. This study showed that the survival of encapsulated culture of L. brevis showed a decline in viable count of about 0.9 log over a period of 4 weeks while there was a decline of about 1.6 log in cultures which were incorporated as free cells in yogurt. |
Year | 2002 |
Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BP-02-06 |
Type | Thesis |
School | School of Environment, Resources, and Development (SERD) |
Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
Academic Program/FoS | Bioprocess Technology (BP) |
Chairperson(s) | Rakshit, Sudip K.; |
Examination Committee(s) | Athapol Noomhorm;Jindal, Vinod K.; |
Scholarship Donor(s) | Royal Thai Government; |
Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2002 |