Method of differentiation of pathogenic bacteria by denaturing gradient gel electrophoresis analysis of PCR- amplified genes coding for ribosomal DNA spacers | |
| Author | Pratima, Vinukonda |
| Call Number | AIT Thesis no.FB-04-12 |
| Subject(s) | Pathogenic bacteria Gel electrophoresis Nucleotide sequence |
| Note | A thesis submitted in partial fulfilment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-04-12 |
| Abstract | Polymerase chain reaction based amplification and denaturing gradient gel electrophoresis were studied to differentiate between pathogenic organisms at genus, species and strain level. A simple and cheap method of DNA extraction by a detergent (Triton X) and test was found to be advantageous as far as DNA amplification is concerned as compared to 5 other methods used. There is not much difference between the methods as far as yield and purity was concerned. The amplified genes in spacer regions of 16Sand 23S rRNA using a universal primer with and with out GC clamp can be used to differentiate organisms was possible. This overcomes the need to have organisms or genes specific primer for each organism. By the use of DGGE method, possible identification and differentiation between genus and strains with a single base pair difference in the DNA sequences were studied. |
| Year | 2004 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-04-12 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Other Field of Studies (No Department) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip K. |
| Examination Committee(s) | Jindal, Vinod K.; Athapol Noomhorm |
| Scholarship Donor(s) | The Royal Netherlands Government |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2004 |