Cryopreservation of silver barb, Barbodes gonionotus sperm using different extracellular cryoprotectants | |
| Author | Naw Deborah |
| Call Number | AIT Thesis no.AQ-05-22 |
| Subject(s) | Fishes--Spermatozoa--Cryopreservation Cryopreservation of organs, tissues, etc. |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-05-22 |
| Abstract | This study attempted to understand the role of extraccllular cryoprotectants in cryoprescrvation trials of silver barb, Barbo(les gonlonolis. As a preliminary test, initial screening of various extraccllular cryoprotectants were conducted. Milk Powder 6% BSA 20% and Glucose 10 % indicated promise. From the final experiment, Glucose 10% had more consistent mean motility 50±0.0 (66.6% of control), mean viability 45.7±0.14 (149% of control) and mean fertilization rate 48.3±0.218 (75.7% of control). None of the treatment hatched except for control. While this study indicates that there might be sonic positive effects of certain extracellular cryoprotectants on the motility, viability and fertilization rates, the experiment needs to he repeated for more reliable results as the quality of both eggs and sperm during this experimental period were less than optimal. |
| Year | 2005 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-05-22 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Bart, Amrit N.; |
| Examination Committee(s) | Yakupitiyage, Amararatne;Ikejima, Kou; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2005 |