1 AIT Asian Institute of Technology

Nitrogen removal from digested piggery waste based on Nitritation-Denitritation process and related microbial community analysis

AuthorGhosh, Gopal Chandra
Call NumberAIT Thesis no.EV-05-10
NoteA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science
PublisherAsian Institute of Technology
AbstractDue to environmentally unfavorable current pig firms management in Asia and, particularly, in Thailand discharged piggery waste usually contains proportionately greater amount of nitrogenous matter that presents a serious challenge for any treatment alternative. This study investigated the nitrogen removal performance of a two-stage Upflow Floating Filter system (in series with recirculation from aerobic stage to anoxic stage) for digested piggery waste based on post-nitritation and pre-denitritation processes under tropical climatic condition. The system was operated continuously for 130 days with constant organic loading rate 0.36kg-N/m 3 of filter.ct, without needing external carbon source for denitritation and without backwashing. As such, treatment of digested Piggery waste becomes possible with a maximum overall removal efficiency of 70% for Total Kjeldahl Nitrogen (TKN), 80% for ammonia nitrogen (NH3-N), and 60% for Total Nitrogen (TN), when running with influent concentrations of 1000 mg/L COD, 250 mg/L TKN and 190 mg/L NH3-N; with a total hydraulic retention time (HRT) 16h, dissolved oxygen (DO) concentration 1.0-1 .5 mg/L in aerobic stage, and recirculation rate 2: 1 from aerobic stage to anoxic stage. Fluorescence in situ hybridization (FISH) and Polymerase Chain Reaction (PCR) were performed to study the nitrogen transforming microbial community, and its variations with time courses. In upflow aerobic floating filter (UAerobicFF), the /3-proteobacteria always represented the majority of the total detected bacteria and most of the f3-proteobacteria were ammonia-oxidizer, but nitrite oxidizer Nitrobacter sp. were rarely found. On the other hand in the upflow anoxic floating filter (UAnoxicFF), a- proteobacteria represented the majo1ity of the detected bacteria, and detected denitrifies community were the substantial portion of a- proteobacteria. FISH and PCR results successfully detected the nitrogen transforming microbial communities, and its variations with time courses in the biofilm, and the results coincided with the reactor performance.
KeywordSewage--Purification--Nitrogen removal ;Sewage--Biological treatment
Year2005
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC))
Academic Program/FoSEnvironmental Engineering and Management (EV)
Chairperson(s)Shipin, Oleg V.;Lee, Seung Hwan ;
Examination Committee(s)Visvanathan, C.;Nguyen Thi Kim Oanh;
Scholarship Donor(s)Government of France;
DegreeThesis (M. Sc.) -- Asian Institute of Technology, 2019


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