Characterization of microbial community of different wastewater treatment ecosystems | |
| Author | Uralov, Bakhodir B. |
| Call Number | AIT Thesis no.EV-05-4 |
| Subject(s) | Microbial populations Sewage--Purification--Biological treatment |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | An environmental awareness has become one of the important parts of human beings life in recent years. In the past, our ancestors were dominated by a power of nature, however, by 20 century, after technological revolution people started to influence negatively to the nature and consequently it has lead to much instability in our mother earth. In this point of view current study was dedicated to solve and learn environmental instabilities via investigating environmentally important microorganisms, their existence, composition and dynamics in the natural as well as in engineering designed wastewater treatment systems. This study mainly concentrated to elucidate microbial community in the different wastewater treatment ecosystems by applying in situ analysis of microbial population. Eleven probes were used to characterize microbial diversity in wastewater treatment systems. In situ analyses were successfully done and composition of autotropic and heterotrophic bacteria showed correlation of the two different wastewater treatment system performances. Investigations of microbial population were done on Upflow Anaerobic Floating Filter (UAnFF) reactor and constructed wetland. UAnFF were at steady state condition by showing approximately 80% COD removal when in situ studies carried out. Lab-scale experiment was prolonged nearly 4 month under constant HRT 24 hours with loading rate 5 kgCOD/m3/d and COD/TKN ratio was 16.3 with having negligible fraction of nitrate nitrogen in the influent. The second study on in situ analysis of microbial community was done at full-scale treatment system - AITs constructed wetland. The system is combined to facultative pond and receives primarily treated effluent with an average COD/TKN ratio of 3.08 of organic matter. The designed HRT of the system is 2.08 days and average loading rate of 61.8 kgTKN/ha.d. During the observation it was found that COD and NH3 were being removed in terms of mass loading with 38.4 and 12.3 % efficiency, respectively. Findings from Florescence In Situ Hybridization revealed different bacteria by representing their diversity of taxanomy and metabolism in different wastewater treatment ecosystems. Aside these, specific probes used such as BET42 (- proteobacteria), NSO1088 (ammonia oxidizers-Nitrosomonas) and ALF1b (common denitrifies) and showed the correlation with physicho-chemical parameters in terms of nitrification efficiency. Whereas, same amount of probes used on UAnFF and it was found that archaea species were dominant over the bacteria by performing COD removal with 78-80 % efficiency. |
| Year | 2005 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering (EV) |
| Chairperson(s) | Shipin, Oleg V. |
| Examination Committee(s) | Annachhatre, Ajit P.;Aramaki, Toshiya;Lee, Seung Hwan |
| Scholarship Donor(s) | Government of Japan |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2005 |