Enzyme immobilization on glass surfaces for the development of biosensors | |
| Author | Sharmin, Farhana |
| Call Number | AIT Thesis no.FB-06-10 |
| Subject(s) | Biosensors Alkaline phosphatase Immobilized enzymes |
| Note | Thesis submitted in partial fulfillment of the requirements for the Degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-06-10 |
| Abstract | Alkaline Phosphatase was immobilized on aminated glass fiber filter disks using covalent bond in the vacuum process. In this procedure, amide bonds were formed between carboxyl groups on the enzyme and amino groups on the glass surface. 10% Glycidoxypropyle trimethoxysilane was the best coupling reagent which could help form bonds between carboxyl and amino group on the glass fiber disk. A 10% concentration of coupling reagent, pH 9.0 and 2 gram of silica were found to be the best conditions for coupling the enzyme over the glass surface showed the highest enzyme activity. Some other parameters (pH and storage activity) were examined and a comparison of immobilized and free enzyme done. The storage life of immobilized enzyme disk was comparably long as compared to as it free enzyme. The covalent attached immobilized enzyme not only retained its activity but also could be reused at least 4 times after washing without loss of enzyme activity. Immobilized enzyme showed nearly 16% loss of enzyme activity after the first trial. An average of 1.65 mg of reusable alkaline phosphatase was immobilized per gram of glass fiber. This immobilized disk was applied as a biosensor. Phosphate elements were measured from water, raw milk and raw shrimp sample by the used of this alkaline phosphatase immobilized disk as a biosensor. Immobilized enzyme can converts substrate to product and then will converts it to a measurable signal. This study demonstrates the possibility of using such a glass disk for the development of biosensors applications. |
| Year | 2006 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-06-10 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Other Field of Studies (No Department) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip Kumar;Athapol Noomhorm |
| Examination Committee(s) | Jayasuriya, Hemanatha P.W. |
| Scholarship Donor(s) | AIT Fellowship |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2006 |