Exploration of potential biocontrol agents against fusarium wilt disease (Fusarium oxysporum f.sp. lycopersici) of tomato (Lycopersicon esculentum) | |
| Author | Kebasen, Steve B. |
| Call Number | AIT Thesis no.AS-07-13 |
| Subject(s) | Biological pest control agents Fusarium wilt of tomato Tomatoes--Diseases and pests |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Agricultural Systems and Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AS-07-13. |
| Abstract | Fusarium wilt disease of tomato, caused by Fusarium oxysporum f.sp. lvcopersici, is one of the major constraints in tomato production in many countries including Thailand. Managing Fusarium wilt by means of pesticides (i.e. Methyl Bromide) has been the most common and widely used method to minimize loss from wilting. However, the detrimental side-effects of the pesticides to humans and the environment are alarmingly demanding the development for safer alternatives for chemical pesticides. Therefore, the study was conducted to explore the possibility for screening and identifying potential antagonist capable of suppressing F. oxysporum f.sp. lvcopersici, the causal organism of Fusarium wilt disease from tomato growing soils and assess their efficacy using in-vivo studies for later incorporating as biological control agents into integrated disease management programs against Fusarium wilt disease. One hundred and eleven isolates, taken from Fusarium wilt disease infected soils and plant samples from Chiang Mai and Kamphaeng Saen had been screened and evaluated, under in-vivo and in-vitro conditions for their potential as biocontrol agents against Fusarium oxysporum f.sp. lvcopersici. Out of 111 isolates, five isolates, coded as KRCI, KRC2, KRC3, KRKU2 and KRKU3 exhibited excellent potential to control Fusarium oxysporum f.sp. lycopersici by reducing its mycelial growth under in-vivo conditions. Results of in-vitro experiments, comparing the efficacy of the selected biocontrol candidates with Trichoderma harzianum, under 5 inoculum levels and two methods of inoculation, confirmed the potential of the selected biocontrol candidates. All the isolates were capable of reducing the incidence of wilting at various degrees. Isolate KRKU3 proved to be superior to all the other isolates and T. harzianum. Moreover, promotion of plant growth by the selected biocontrol candidates had been observed at various extents. Root dipping method was found as the better method of inoculation, which also promoted plant growth and development. On the other hand, direct inoculation of the biocontrol agents was observed to promote significantly greater control for wilting caused by Fusarium oxysporum f.sp. lycopersici in soil. |
| Year | 2007 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AS-07-13 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Agricultural and Aquatic Systems (AS) |
| Chairperson(s) | Ranamukhaarachchi S.L.; |
| Examination Committee(s) | Shipin, Oleg 0.;Clemente, Roberto S.; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2007 |