Assessment of effect of aniline on the biological nitrification process using FISH technique | |
| Author | Nguyen Phuc Thanh |
| Call Number | AIT Thesis no.EV-07-29 |
| Subject(s) | Fluorescence in situ hybridization Aniline |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Environmental Engineering Management. |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. EV-07-29 |
| Abstract | Complete nitrification was investigated with synthetic wastewater containing high ammonium nitrogen concentration and low COD by the activated sludge as a chemoautotrophic process in the Continuously Stirred Tank Reactor (CSTR). Seed sludge was taken from rotating biological contactors of a domestic wastewater plant. The process was operated under the temperature of 27 - 30°C, pH in a range of 7.2 - 8.0, hydraulic retention time of 5 hours, sludge retention time of 12 days and DO was maintained at the value more than 4.0 mg/L. Complete nitrification was successfully achieved with a NH⁴4-N loading rate of 1.6 kg/m3.d. Then inhibition of complete nitrification process by adding aniline step wise was studied. It was found that nitrification process started to be inhibited at aniline concentration of 20 mg/L. Ammonium removal efficiency was decreased in a range of 6 - 8 %, the NO2N formation efficiency was increased around 0.6 - 1.5 %, and the NO3N formation efficiency was also fell in a range 0.2 - 1.9 %. Inhibition of aniline on nitrite oxidation is more than on ammonia oxidation. FISH technique was used to study the nitrifier population shift for complete and inhibited nitrification. It confirmed that inhibitor aniline affected to nitrifying microorganism. Population of nitrifier was reduced strongly as compared with samples taken during complete nitrification. And aniline affected to the reduction of Nitrite oxidation bacteria population more than that of Ammonium oxidation bacteria. |
| Year | 2007 |
| Corresponding Series Added Entry | Asian Institute of Technology.Thesis ; no. EV-07-29 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering (EV) |
| Chairperson(s) | Annachhatre, Ajit P.; |
| Examination Committee(s) | Chongrak Polprasert;Thammarat Koottatep; |
| Scholarship Donor(s) | MOET, Vietnam;AIT Fellowship; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2007 |