Development of antimicrobial photodynamic poly (3-hydroxybutyrate-co-3-hydroxyvalerate) packaging film for food preservation | |
| Author | Truong Dang Le |
| Call Number | AIT Thesis no.FB-20-02 |
| Subject(s) | Photosensitization, Biological Antimicrobial polymers Packaging Food |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no.FB-20-02 |
| Abstract | Overuse of plastic packaging has caused serious pollution for environment and chemical additives for food preservation are harmful for human health. This study investigated the incorporation of selective photosensitizer into biodegradable polymeric film which is exposed to blue Light Emitting Diode (LED) for developing novel biodegradable antibacterial packaging film on the surface of food materials. This bactericidal packaging film is based on the photodynamic treatment (PDT) when the photosensitizer (PS) compound absorbs the light energy then produces reactive oxygen species (ROS) for bacterial inactivation. Three natural PS including curcumin, aloe emodin and astaxanthin were employed in this study for selection of the most effective compound for PDT. Aloe emodin (AE) was considerably the most efficacy which reduced 2.8 and 1 log CFU/cm2 of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) population when it was applied the absorbed photon number of 4.72 x 1017 and 2.36 x 1018 photons/cm2 , respectively. Whereas, curcumin (CUR) only reduced 0.5 log CFU/cm2 of S. aureus and no inhibitory effect on E. coli at the same absorbed photon number used for AE. In addition, there was no observation of for astaxanthin (AST) in phototoxic effect against these bacteria. AE was selected for incorporation into poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) polymer for developing packaging film (AE-PHBV film). The AE concentration of 2 µmol per gram of PHBV in the film showed the highest cytotoxic efficacy in reducing nearly 5 log CFU/cm2 of E. coli population under radiant fluence of 21.6 J/cm2 . Nanocellulose crystal (CNC) was used as reinforcing agent for enhancing mechanical properties of AE-PHBV film. CNC with 2% (w/w) compared to PHBV in the film increased by 122.5% and 158% in tensile strength and Young’s modulus while remaining antibacterial efficacy of neat AE-PHBV film. |
| Year | 2020 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no.FB-20-02 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Loc Thai Nguyen; |
| Examination Committee(s) | Anal, Anil Kumar;Bora, Tanujjal; |
| Scholarship Donor(s) | AITCV Silver Anniversary Scholarships; |
| Degree | Thesis (M. Eng.) - Asian Institute of Technology, 2020 |