Formulation optimization for preparation and characterization of chitosan-peptide nanoparticle from poultry feather | |
| Author | Chokpipat Wonglamai |
| Call Number | AIT Thesis no.FB-20-06 |
| Subject(s) | Chitosan Protein hydrolysates Bioactive compounds |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-20-06 |
| Abstract | This research is aimed to investigate the production of chicken feather protein hydrolysate using keratinase enzyme and determine the optimization conditions for the hydrolysis process. Moreover, the keratin hydrolysate was further utilized to develop the chitosan-peptide nanoparticles. The modification was conducted to improve the antibacterial activity, antioxidant activity and stability compare to the unmodified protein hydrolysate. For the enzymatic hydrolyzation process, the enzyme activity and hydrolyzation time was varied. As a result, the enzyme activity at 6,900 U and time at 6 h was designed as the optimum condition for feather hydrolyzation using keratinase enzyme. For the characterization of developed hydrolysate, the antioxidant activity exhibited from the feather keratin hydrolysate which is higher than cosmetic grade keratin hydrolysate at 60.53 ± 3.2 and 39.82 ± 2.2 % respectively at concentration of 1 mg/mL. However, the hydrolysate produced from the experiment do not exhibit any antibacterial activity after tested with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) evaluations. The Chitosan peptide nanoparticles (CPNs) was successfully developed using cosmetic grade keratin hydrolysate with the size approximately around 90 -130 nm. For the nanoparticle formation, the optimized conditions to develop the nanoparticles are pH and Chitosan: tripolyphosphate ratio at the 5 and 5:1 respectively with average diameters equal to 111.1 ± 0.46 nm. The antioxidant activity of the developed CPNs which encapsulated the feather keratin hydrolysate for 4 mg/ mL is equal to 44.88 %. In addition, the storage stability test was conducted. The size of CPNs storage at pH 2 and 4 for 7 days can remain stable while the denaturation was occurred when submerge in the buffer solution pH 6. The antioxidant activity of CPNs storage at pH 2 exhibit the highest antioxidant activity. |
| Year | 2020 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no.FB-20-06 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Loc Thai Nguyen;Bora, Tanujjal; |
| Scholarship Donor(s) | Bangchak Petroleum Public Company Limited, Thailand;Royal Thai Government Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2020 |