Optimazation of ligh conditions to improve the growth of cordyceps militaris and development of encapsulation system to enhance its stability | |
| Author | Tu Trinh Thi Nguyen |
| Call Number | AIT Thesis no.FB-20-09 |
| Subject(s) | Cordyceps Encapsulation Growth |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no.FB-20-09 |
| Abstract | In this study, various intensities of light and chroma were evaluated for the growth and productivity of Cordyceps militaris. The effects of light on productivity and bioactive compounds in the fruiting body of Cordyceps militaris were determined. Treating by white light first, and then red and blue combine at 500 lux in the production of Cordyceps militaris process was the optimal condition with the figure of 9.37 ± 0.32 cm height of fruiting bodies, 5.47 ± 0.15 g dry weight of fruiting bodies per cup. For quality of Cordyceps militaris, cordycepin, polysaccharide, phenolic, flavonoid content in fruiting body were determined with the figure of 7.96 mg/g (d.w), 17.03 ± 0.35 mg/g (d.w), 65.57 ± 0.33 (GAE mg/g), 30.61 ± 0.44 (QE mg/g), respectively, by using spectrophotometric and HPLC method. Besides, phenolic and flavonoid content determination was different because of solvent extract influence. The result of this study indicated water was the best choice about solvent used to extract phenolic and flavonoid from Cordyceps militaris. On the other hand, the consequence of encapsulation on a bioactive compound in Cordyceps powder was presented distinctly through temperature and pH stability of encapsulated powder with different ratio mixing (core and coat material). E20 as an encapsulated powder with a mixing ratio of 1:20 w/w (Cordyceps powder: Maltodextrin) was the highest stability under heating and acid condition. Phenolic content trapped in E20 matrix was 14.55 ± 0.37 mg/g at 37 °C, 14.18 ± 0.28 mg/g at 60 °C, 13.89 ± 0.33 mg/g at 70 °C, 11.61 ± 0.46 mg/g at 80 °C and 11.46 ± 0.53 mg/g at 90 °C. Also, pH significantly affected phenolic content in E20 with the figure of 8.63 ± 0.1 mg/g at central (pH=7), 8.28 ± 0.1 mg/g at slight acid (pH=6), 7.77 ± 0.14 mg/g at more acid (pH=4) 7.17 ± 0.23 mg/g at strong acid solution (pH=2) and 8.16 ± 0.15 mg/g at base solution (pH=8). Encapsulation of Cordyceps powder by maltodextrin (1:20 ratio) was high efficiency with the EE (%) figure of 95.96 %. |
| Year | 2020 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no.FB-20-09 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Loc Thai Nguyen;Salin, Krishna R.; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2020 |