Probiotic lactobacillus casei release from calcium alginate beads coated with chitosan | |
| Author | Khamsing Louangsihalath |
| Call Number | AIT Thesis no.FB-07-16 |
| Subject(s) | Lactic acid bacteria Lactobacillus casei |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-07-16 |
| Abstract | The main aim of this work was to study the use of chitosan coated alginate beads with immobilized probiotic Lactobacillus casei as an approach to controled cell release in the stomach and other appropriate systems. The effect of chitosan concentration coating on the release of microorganisms from alginate beads, the effect of one layer and two layers of chitosan on alginate beads, the effect of intracapsular cell population on initial cell release was evaluated. Lactobacillus casei TISTR 390 was used as a model probiotic for this study. It was found that initial release of microorganisms from coated beads was the same as uncoated beads, but later coated beads released smaller numbers. Hence pH changes and cell numbers were finally lower with uncoated beads. When cells were released into milk it was difficult to account for the changes, as the number in the milk was dependant on release and subsequent growth. But in the experiments with release into water release was a function of the concentration of chitosan used for coating. With chitosan coating on alginate beads there was a decrease in weight with an increase in the concentration of chitosan and an increase in release from the beads was obtained. The alginate beads were protected better with coating using higher concentrations of chitosan (1.6%) when immersed in solution of pH 1.5. Double layer of chitosan restricted release of free cells more than single coating. However after eight hours the amount of release was the same. The release of microorganisms from the beads was dependant on the concentration of microorganisms in the beads. Higher the cell concentration in the beads higher the release of cells. However there was a significant difference in cell numbers depending on whether the beads were coated or not coated. Overall, depending on the need, the amount and rate of release of probiotic can be controlled using different concentration of chitosan and level of coating. |
| Year | 2007 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-07-16 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Ranamukhaarachchi, S.L.; |
| Scholarship Donor(s) | Sweden (Sida); |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2007 |