Production of nanoscale cellulose from rice straw by the fermentation of Trichoderma reesie | |
| Author | Wuthichai Jomnui |
| Call Number | AIT Thesis no.FB-20-13 |
| Subject(s) | Cellulose--Biotechnology Nanocomposites (Materials) Fermentation |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no.FB-20-13 |
| Abstract | Agricultural waste like cellulosic waste is one of the wastes that hard to utilize due to the structure of cellulosic waste which is hard to react with chemicals or enzymes. Rice straw is main cellulosic waste in Thailand. It has a trend to be increased every year, while the potential to utilize is low around 10% of waste. This research aims to use rice straw as a substrate for fermentation by Trichoderma reesei to convert rice straw to nano-cellulose. It dived into two sections that pretreatment and production. Pretreatment is processing to prepare rice straw before using. Alkali and hydrogen peroxide treatments are methods to compare which is a suitable treatment. The result showed that both methods which are mixed alkali (0.5% of KOH, 2% of Ca(OH)2) and 3% of hydrogen peroxide were significantly reduced lignin component which is an issue in fermentation from 37% to 17% and 30%, respectively. The production of nanocellulose was operated by T. reesei which has cellulase to convert cellulose to small molecules. From the result, T. reesei can converted treatment rice straw to nanocellulose and the yield of nano-cellulose is 0.88 and 0.32 % from alkali treatment rice straw and hydrogen peroxide treatment rice straw, respectively. To confirm nanocellulose, FTIR and TEM were used to confirm. The FTIR results were confirmed that the product from fermentation is nan-cellulose. TEM was used for measuring the size that showed nano-cellulose from alkali treatment rice straw and hydrogen peroxide treatment rice straw that are 500 nm and 1000 nm, respectively. To achieve this fermentation, it spends around $20 or 660 baths for 1L fermentation and will get nanocellulose around 1.76 g. |
| Year | 2020 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no.FB-20-13 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Loc Thai Nguyen; |
| Examination Committee(s) | Anal, Anil Kumar;Datta, Avishek; |
| Scholarship Donor(s) | Royal Thai Government Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2020 |