Estrogenic activities of sesame lignans and their metabolites : interactive effect with cadmium in human breast cancer cell lines | |
| Author | Prisna Pianjing |
| Call Number | AIT Diss. no.EV-11-04 |
| Subject(s) | Seseme Estrogen Breast--Cancer |
| Note | A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | The estrogenic activities of sesame lignans and their metabolites were investigated by
using a viability assay and estrogen response elements (ERE) rep01ier system on the
different levels of estrogen in cultured media. Hormone-dependent human breast cancer
T47D cells and the cells stably transfected with ERE-luciferase reporter system T47DKBluc cells were utilized. Depending on levels of E2, sesame lignans and their metabolites
exhibited different effects on the viability of T47D-KBluc cells. In the absence of
estrogen, the trend of induction of cell growth was observed, whereas in the presence of
estrogen, cytotoxic effect was evidenced. The ERE luciferase rep01ier assay in T47DKB1uc cells shows that, sesame lignans and their metabolites induced ERE activation.
Among tested compounds, sesamol possessed the highest ERE activation prope1iy while
ED showed no effect. All tested compounds exhibited lower levels of ERE activation than
that of estradiol (E2) activation, suggesting that the tested compounds possess weak
estrogenic effect. The antiestogen ICI 182 780 significantly decreased ERE activation
induced by the te |
| Year | 2011 |
| Type | Dissertation |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Jutamaad Satayavivad ;Apinya Thiantanawat ; Preeda Parkpian |
| Examination Committee(s) | Nuchanart Rangkadilok ;Bung-om Sripanidkulchai |
| Scholarship Donor(s) | Chulabhorn Research Institute/Mahidol University/ AIT Fellowship (CRI-MU-AIT) |
| Degree | Thesis (Ph.D.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2011 |