Chitosan effect on plant tissue culture | |
| Author | Khin Lay Nge |
| Call Number | AIT Thesis no.BT-03-13 |
| Subject(s) | Chitosan Plant tissue culture |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. BT-03-13 |
| Abstract | Chitosan plays an important role in plant growth regulation. This ability has been investigated in this study using the plant tissue culture technique.In plant tissue culture plants of interest can be multiplied in a short period of time. Biostimulation activity by chitosan can be demonstrated by qualitative and quantitative effects on the growth rate and the properties of the cultured plants. The plant tissue culture is also very appropriate to compare the bioactivity of various molecular forms of chitosan and to study the effect of various concentrations of these chitosans. Different molecular weight of chitosan, 100 k Da, 10 k Da and oligomer were investigated in orchid meristem culture using liquid media. Oligomer and 10 k Da chitosan was most effect on protocorm like body formation. Different concentration of (5, 10, 15, 20 and 25) ppm of shrimp and fungus chitosan (lOkDa) and oligomer chitosan were investigated in propagation of protocorm in liquid media and on agar media by culturing orchid protocorm. The result showed that the 15-ppm was the specific concentration on orchid plant tissue culture in liquid media and 20 ppm was the specific concentration on plant growth rate of orchid protocorm on agar media. Fungus polymer chitosan was the best effect of growth regulator among three kinds of chitosan. The next experiments were the investigation of chitosan effect on com and bell pepper culture to determine the specific concentration of chitosan for agar media. The result showed that the 20-ppm was specific concentration for only chitosan effect and 5 and 10 ppm were specific concentration for chitosan plus hormone effect. |
| Year | 2003 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BT-03-13 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Stevens, Willem F.; |
| Examination Committee(s) | Suwalee Chandrkrachang;Athapol Noomhorm; |
| Scholarship Donor(s) | Asian Institute of Technology; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2003 |