| Author | Pham Huong Lien |
| Call Number | AIT Thesis no.FB-05-12 |
| Subject(s) | Lectins
|
| Note | A thesis submitted in pa1tial fulfillment of the requirements for the
degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-05-12 |
| Abstract | Lectin was isolated from the Abrus precatorius seeds by Ammonium sulfate precipitated, dialysis and Sepharose-4B affinity chromatography. The homogeneity of the lectin was demonstrated by SDS-PAGE in the presence of P-mercaptoethanol. Only one protein band with an apparent molecular mass of 60 kDa was found. The Abrus precatorius lectin showed carbohydrate specificity for D-galactose, a requirement for divalent metal rations (Ca2+ and Mn2+) for full activity. The hemagglutination activity was found to he stable up to 85°C and more stable under alkali than acid condition. Ouchterlony lmutnunodiffusion assays using human antiserum raised against the purified lectin of Abrus precatorius showed that it was possible to detect only IgA 1. The purified lectin was carried out to identify coaglulase-negative and coaglulasepositive staphylococci, Bacillus thuringiensis, Bacillus antluacis and Listeria monocytogenes serotype 3a. IgAl concentration was found to be higher in patients with liver cancer and hepatitis B compare to a value of 2.84 mg/ml in healthy person by using Enzyme - Linked -- Lectinosorbent - Assay (ELLA) method. Abrus precatorius lectin thus has a specific reaction with antibody IgAl and can be used for diagnosis |
| Year | 2005 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-05-12 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip K.; |
| Examination Committee(s) | Athapol Noomhorm ;Jindal, V. K.; |
| Scholarship Donor(s) | Government of the Netherlands; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2005 |