Enzyme assisted starch extraction from cassava roots | |
| Author | Kyawt Kay Kay Khine |
| Call Number | AIT Thesis no.FB-05-22 |
| Subject(s) | Starch Cassava |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-05-22 |
| Abstract | The starch extraction from Cassava root can be extracted by biochemical methods. High recovery of starch can be obtained by using commercial cell wall degrading enzymes in biochemical starch extraction. Starch yield can be improved by optimizing the production parameters of enzyme assisted extraction. In this study, the experiments were carried out to study the effect of the production parameters of different type of enzyme: Celluclast, Multifect, Pectinex, and their combination. The parameters tested were pH, temperature, incubation time and enzyme concentration. Firstly the optimum condition of individual enzyme was tested. The optimum condition of Celluclast was pH 4, temperature 45°C, enzyme concentration 0.5%, incubation time 6 hours and 100 rpm and it gave 74% starch recovery. The optimum condition of Pectinex was pH 3.5, temperature 35°C, enzyme concentration 0.5%, incubation time 6 hours and 100 rpm. The optimum condition of Multifect was pH 5.5, temperature 50°C, enzyme concentration 0.5%, incubation time 6 hours and 100 rpm, and it gave 79.47% starch recovery. Although the starch recovery of Celluclast was not different from that of Pectinex, the starch recovery of Celluclast was much better than that of Pcctincx. The production parameters used in starch extraction by Celluclast and Pectinex combination was the optimum condition of Celluclast obtained from early experiment. The combination of 0.5% Celluclast and 0.1% Pectinex could increased starch recovery up to 38.17% compared to same operation condition without enzyme. The production parameters used in starch extraction by Multifect and Pectinex combination was the optimum condition of Multifect obtained from early experiment. The combination of 0.5% Multifect and 0.1% Pectinex could increased starch recovery up to 41.21% compared to same operation condition without enzyme. According to the experimental results, it can be concluded that the effect of enzyme combination was better than the enzyme alone. The combination effect of Multifect and Pectinase was better than that of Celluclast and Pectinex. Therefore, the combination of 0.5% Multifect enzyme and 0.1% Pectinex enzyme can be recommended to use in enzyme assisted starch extraction. |
| Year | 2005 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-05-22 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Athapol Noomhorm; |
| Examination Committee(s) | Jindal, Vinod Kumar ;Jayasuriya, H. P. W.; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2005 |