| Author | Budi Prakoso |
| Call Number | AIT Thesis no.AC-99-28 |
| Subject(s) | Postharvest diseases and injuries
|
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AC-99-28 |
| Abstract | Natural microorganisms can provide an effective prevention of the decay of fruits and vegetables that occurs post harvest due to pathogenic action of plant pests. The development and use of microorganisms as biological control agents should be encouraged as one alternative of chemical pesticides. However, the possible application of microorganism on fruits and vegetables should be assessed carefully since they can have an undesirable impact as well on human health and environment. Reliable identification and enumeration methods are needed for knowing the survival, multiplication and abundance of the biological control agent to ensure that the biological control agent (BCA) itself can be kept under control and applied safely. In this study the enumeration of the candidate biological control agent Debaryomyces hansenii strain 0 has been studied. Strain 0 can reduce or delay tissue rottening in apples caused by Botrytis cinerea, whereas 10 other strain of the same species are not active in this respect. For its use as BCA, an efficient method to distinguish between strain 0 and those other 10 strains should become available. Detection by the Polymerase Chain Reaction (PCR) using Random Amplified Polymorphic DNA (RAPD) is one of the methods for identifying microorganisms. This method has been used in this research for identification of a natural DNA marker of strain 0 . Based on the sequence of the identified DNA marker, primers have been designed for probing the DNA marker. Results show that RAPD can differentiate strain 0 from 10 other strain belonging to the same species, Debaryomyces hansenii. Only one pair of primer combination, BPRKS 1 and BPRKS3, out of 8 pairs can be used for identifying strain 0 on surface-sterilized and non sterilized apples within 24 hour after application of the strain 0 on the apple surface. PCR technique alone can only identify the occurrence of strain 0. Combining plate counting and PCR techniques allows enumeration of strain 0. |
| Year | 1999 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AC-99-28 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Agricultural, Conservation and Rural Development (AC) |
| Chairperson(s) | Hansen, Gunner K.; |
| Examination Committee(s) | Stevens, Willem F. ;Athapol Noomhorm; |
| Scholarship Donor(s) | Asian Development Bank; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1999 |