Interaction between cassava starch, lipid and chitosan | |
| Author | Ding, Lian |
| Call Number | AIT Thesis no. BP-99-05 |
| Subject(s) | Lipids Chitosan Cassava industry |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | Mixtures of Cas~ava starch, oil, monoolein, or rapeseed fatty acid were stirred heated in Rapid Visco Analyzer (RVA). Properties of the mixture including peak viscosity, final viscosity, breakdown, trough, pasting temperature, peak time etc. were measured in RVA first profile. Thereafter, non-specific lipase was added to the gel of the mixture and stirred heated in RVA undergoing second profile. The mixture after enzymatic hydrolysis in RVA was extracted by hexane in order to extract lipids, which are the hydrolysis products. The hexane extracts were taken to analysis by using TLC and titration. Lipids changed physicochemical properties of Cassava starch. Peak and final viscosity decreases with presence of lipids with the exception that final viscosity increases with presence of mono-olein. Lipids were complexed with Cassava starch in RVA, which blocked the lipase hydrolysis. Chitosan was mixture with oil in emulsifier. Then the mixture was hydrolyzed by nonspecific lipase. The acid value after hydrolysis was obtained by titrating the hydrolasate of the mixture with KOH in presence of ethanol/ether (l: 1). Less acid value was obtained by titration with increasing quantity of chitosan, which indicated the inhibition of chitosan on oil hydrolysis. ln different buffer of which pH value is 4, 5, 6, 7, 8, and 9, the titration of chitosan/oil mixture is interfered, which inferred the instability of chitosan in different acidic medium. |
| Year | 1999 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Montet, Didier |
| Examination Committee(s) | Stevens, Willem F.;Suwalee Chandrkrachang;Rakshit, Sudip Kumar;Pakorn Nuchnoi |
| Scholarship Donor(s) | Asian Institute of Technology |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 1999 |