Deproteinization of shrimp bio-waste by fermentation, enzymes and chemicals and analysis of the protein fraction | |
| Author | Kyaw Nyein Aye |
| Call Number | AIT Thesis no. BP-98-3 |
| Subject(s) | Fermentation Industrial microbiology Shrimp industry--Waste disposal |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | Fermentation of shrimp bio-waste refers to lactic acid bacteria fermentation that can hydrolyze the protein from shrimp heads and shells. The hydrolysate obtained in this process is a rich source of protein besides calcium, astaxanthin and other minerals. The protein fraction was analyzed. Fermentation by lactic acid bacteria is one of the alternatives to the chemical method for deproteinization of shrimp bio-waste. Chemical methods require strong acid and alkali, which tend to pollute the environment. Moreover, these chemicals degrade the protein, thus losing valuable by-product. The fermentation method can overcome such problems. Lactic acid bacteria, Lactobacillus plantarum can optimize the needs of environmental and economical benefit on utilization of shrimp bio-waste. Protease enzymes were also studied in combination with fermentation. Comparison among the treatments of lactic acid bacteria fermentation, bacteria combined with enzyme and protease enzyme only was conducted. Hydrolysate was separated from solid residue. Protein content determination was done for hydrolysate and residue. Glucose was used as an additional substrate for the bacteria. Substitution of other substrates could be compared with glucose. Citric acid and lactic acid addition could replace acetic acid in the presence of lactic acid bacteria. Various techniques of production of shrimp protein powder were investigated to evaluate the product quality. |
| Year | 1998 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Suwalee Chandrkrachang ; |
| Examination Committee(s) | Stevens, Willem F. ;Rakshit, Sudip K. ;Pakorn Nuchnoi ;Montet, Didier ;Attaya Kungsu an |
| Scholarship Donor(s) | His Majesty King Bhumibol Adulyadej of Thailand; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 1998 |