Partial purification and characterization of transglutaminase produced by Streptoverticillium Ladakanum | |
| Author | Li, Ping |
| Call Number | AIT Thesis no. BP-98-6 |
| Subject(s) | Biochemical engineering Transglutaminases |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | Transglutaminase (TGase) from Streptoverticillium ladakanum was purified by three purification processes after filtration. The first process consisted of ammonium sulfate fractionation, Amberlite CG-50 ion exchange chromatography and Sephadex G100 gel filtration chromatography. The second purification process consisted of ammonium sulfate and Sephadex G-100 chromatography while the third process consisted of Amberlite CG-50 chromatography only. Comparing these three purification processes, the second process was found to be the best method for purification of transglutaminase from Streptoverticillium ladakanum. The molecular weight of the TGase was 32.5 kDa, estimated by Sephadex G-100 gel filtration. The enzyme activity was maximum at pH 5.4-7.5 and temperature at 40-50°C. It was also found that the enzyme was stable at pH range of 5-8 and temperature up to 50°C. The TGase activity was strongly inhibited by Zn2 + and Cu2 +, but not by Mg2 +, K+, Ca2 + and EDTA. |
| Year | 1998 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Pakorn Nuchnoi; |
| Examination Committee(s) | Stevens, Willem F. ;Rakshit, Sudip K. ;Suwalee Chandrkrachang ;Montet, Didier; |
| Scholarship Donor(s) | HM King of Thailand; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1998 |