Separation of amylose and amylopectin fractions from cassava starch | |
| Author | Shagdar, Narantuya |
| Call Number | AIT Thesis no. BP-98-9 |
| Subject(s) | Starch Tapioca |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resource and Development |
| Publisher | Asian Institute of Technology |
| Abstract | Commercial cassava starch with the trade name "The best tapioca starch" was used in the fractionation experiments of amylase and amylopectin. Before the fractionation experiments the physico-chemical properties of native cassava starch were determined in terms of moisture content, ash content, amylase content, acidity, viscosity, reducing sugar, and total sugar. Amylase and amylopectin were fractionated from cassava starch by two different methods: chemical method using butanol as complexing agent, and enzymatic method producing allamylose starch with promozyme (debranching enzyme). The purity of the fractionated products was determined by HPSEC analysis. Results of these experiments and analysis show that the chemical method of fractionation was most suitable for separation of amylopectin. The purity of the fractionated amylopectin by this method was 82-91.5%. By contrast, the purity of amylase obtained was 51-61 %. Results of the experiments with debranching enzyme-promozyme show that, this method is very convenient and effective for production of amylase from cassava starch. Purity of amylase produced by this method was 88%. |
| Year | 1998 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Suwalee Chandrkrachang ;Stevens, Willem F.; |
| Examination Committee(s) | Rakshit, Sudip Kumar ;Pakorn Nuchnoi ;Montet, Didier ; |
| Scholarship Donor(s) | Japanese Government Asian Development Bank; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1998 |