| Author | Varaporn Burananon |
| Call Number | AIT Thesis no. BP-98-10 |
| Subject(s) | Oligosaccharides
|
| Note | A thesis submitted in partial fulfillment of the requirements
for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | Immobilization of concentrated glucosyltransferase enzyme on sodium alginate and copolymer of alginate/chitosan complex were studied. About 15-20% and 30% of
immobilization efficiency were observed from immobilization by sodium alginate and
alginate/chitosan complex, respectively. Suitable conditions for preparation of calcium
alginate beads were 3% sodium alginate, 0.2 M CaC12 and incubation time of 1 h with 3 mm
diameter of beads.
Immobilized glucosyltransferase expressed better characteristics (i.e. pH stability or
thermal stability) than free enzyme especially in the case of calcium alginate beads. Calcium
alginate beads had greater stability than chitosan-coated calcium alginate beads and higher
percentage panose production efficiency. However, chitosan-coated calcium alginate beads
had higher storage stability and presented more enzyme activity than calcium alginate beads
when the same amount of enzyme was immobilized. In batch reactor, 45.96% and 33.33 %
panose production efficiency were obtained by calcium alginate beads and chitosan-coated
calcium alginate beads respectively with 5% maltose as substrate. |
| Year | 1998 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Rakshit, Sudip Kumar; |
| Examination Committee(s) | Stevens, Willem F. ;Suwalee Chandrkrachang ;Montet, Didier ;Pakorn Nuchnoi ; |
| Scholarship Donor(s) | Government of the United Kingdom; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1998 |