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The effect of processing methods on the functional properties of whey protein concentrates and their applicability as a food ingredient | |
Author | Dias, Palika Namali |
Call Number | AIT Thesis no. AE-97-7 |
Subject(s) | Whey products |
Note | A thesis proposal submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources & Development |
Publisher | Asian Institute of Technology |
Abstract | This study was carried out to investigate the relationship between processmg conditions and the functional properties of the protein in whey. Whey protein concentrates were prepared from cottage cheese whey by coagulating with chitosan and concentrating by Rotavapor. The best chitosan concentration dosage investigated to coagulate whey was 60 mg/L and the working conditions of Rotavapor to concentrate whey was 90 - 100 RPM and 90-100 mbar vacuum pressure. The four samples obtained from different methods, Rotavapor vacuum dried (RV), Rotavapor Freeze dried (RF), Chitosan vacuum dried (CV), Chitosan freeze dried (CF) and the commercial sample (COM) which was produced by ultrafiltration followed by freeze drying, were investigated for their functional properties . All the samples had good solubility, foaming, gelation, emulsification, Water Binding Capacity (WBC), and Oil Binding Capacity (OBC), but had low viscosity in 6% dispersions and were unable to form stable foams. The functionality among the samples were significantly different (p<0.05), and suggested that there is an effect of processing method on functional properties. RV sample had highest foaming capacity, and OBC, but the solubility, emulsion activity, and gelation was highest in COM sample. Chitosan coagulated samples had the least functionalities except in WBC, and suggested that though it was present in minor amount, the presence of chitosan in the sample had a significant effect (P<0.05) on its functional properties. It also suggested that sugars and salts coprecipitated with the whey protein. The variation in functionality was ascribed to composition of whey protein sample, the ratio of different whey proteins present, and conformation and charges of protein molecules prepared by different methods. |
Year | 1997 |
Type | Thesis |
School | School of Environment, Resources, and Development (SERD) |
Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
Academic Program/FoS | Agricultural and Food Engineering (AE) |
Chairperson(s) | Rakshit, Sudip K.; |
Examination Committee(s) | Jindal, V. K. ;Athapol N. ;Vincent , J. C. |
Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1997 |