A study on the methods of detecting anatoxin-a | |
| Author | Khin Thida Linn |
| Call Number | AIT Thesis no.EV-97-26 |
| Subject(s) | Drinking water--Microbiology Water--Pollution--Toxicology |
| Note | A thesis submitted in partial fulfilment of the requirements for the degree of Master of Science |
| Publisher | Asian Institute of Technology |
| Abstract | A sensitive, simple, reliable and faster extraction method could be developed for the determination of anatoxin-a in the algae suspension water and freeze-dried cell. The excellent linearity with the correlation coefficient over 0.99 with both mobile phase solutions could be drawn using standard anatoxin-a. With 70%methanol+30%0.01M HCIO4 mobile phase solution and 1.2 ml/min flow rate, HPLC could detect the least toxic concentration of 0.34 mg/l (0.0034 µg/10µ1) and with methanol-water (6:4) mobile phase solution and 1 ml/min flow rate, HPLC could detect the least toxic concentration of 0.33 mg/l (0.0033 µg/10 µl). Combined with the extraction procedure which affected a 10-fold concentration and an overall assay sensitivity of 0.03 mg/l (0.3 ng/10µl) was achieved. HPLC with methanol-water (6:4) mobile phase solution, 1 ml/min flow rate could detect the highest amount of anatoxin-a combined with the developed extraction method (M.AA method). Anatoxin-a could be detected from the three species of the cyanobacteria namely Oscillatoria obscura TISTR 8245, Cylindrospermum TISTR 8158 and Cylindrospermum TISTR 8216 as a new investigation. |
| Year | 1997 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Ishibashi, Yoshinobu; |
| Examination Committee(s) | Annachhatre, Ajit P.;Preeda Pakpian; |
| Scholarship Donor(s) | Asian Institute of Technology (Partial Scholarship) ; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1997 |