Formulation of Bio polymeric Multi-layered Encapsulation System for Enhancing the Stability and Controlled Release of Astaxanthin | |
| Author | Dissanayaka, Dissanayaka Mudiyanselage Erandathi |
| Call Number | AIT Caps. Proj. no.BSE-16-06 |
| Subject(s) | Astaxanthin Simulated gastric fluid Sodium alginate |
| Note | A capstone project report submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Engineering Biosystems Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Caps. Proj. ; no. BSE-16-06 |
| Abstract | The aim of the study was to develop a method to enhance the controlled release and the storage stability of astaxanthin. Astaxanthin is a carotenoid compound which possess high anti oxidation properties. The compound is present in sources like algae, flesh of fish (salmon), shells of crustaceans and in feathers of some birds as a red color pigment. The astaxanthin extracted from Haematococcus pluvial was used for this research and encapsulated using the gelation method. The polymers used for encapsulation aiming the controlled release of this antioxidant were sodium alginate and chitosan due to the significant properties they possess enhancing the controlled release of astaxanthin. Three types of beads were made with varying compositions of, sodium alginate, chitosan and CaCl2. Type S beads were encapsulated only with alginate by coagulating in CaCl2, M1 beads contained chitosan in the coagulation fluid together with CaCl2 and post coagulation treatments were also carried out in chitosan. For the M2 type bead there were no chitosan mixed in the coagulation fluid but was treated with chitosan, alginate and CaCl2 in the post coagulation treatments. The beads were characterized mainly for swelling in SGF, disintegration and for the release behavior in SGF and SIF. Further more stability tests were done to the three types of beads over a period of four weeks under four different environmental condition to test the antioxidation levels of encapsulated beads. S1 type showed very less results for the expected outcomes, while the M1 and M2 beads showed favorable outcomes towards the controlled release of astaxanthin. During the stability test it was observed that S type beads showed very less stability and M2 type multilayer beads showed the maximum stability under all four conditions. Also, the most suitable storage condition recorded as the dark cold conditions over a temperature range of 2-5C. |
| Keyword | Astaxanthin, ionic gelation, Simulated Gastric Fluid, Simulated Intestinal Fluid, sodium alginate, chitosan, multi-layer encapsulation, stability. |
| Year | 2016 |
| Corresponding Series Added Entry | Asian Institute of Technology. Caps. Proj. ; no. BSE-16-06 |
| Type | Capstone Project |
| School | School of Environment, Resources, and Development |
| Department | Other Field of Studies (No Department) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Loc Thai Nguyen;Soni, Peeyush;Datta, Avishek; |
| Degree | Capstone Project (B.Sc.)-Asian Institute of Technology, 2016 |