Isolation of Probiotics from Nile Tilapia (Oreochromis niloticus) Gut and Evaluation of their Antagonistic Activities Against Food-borne and Fish Pathogens | |
| Author | Ludowyke, Natali Rose |
| Call Number | AIT Caps. Proj. no.BSE-16-10 |
| Subject(s) | Lactic Acid Bacteria Antagonistic effect Nile Tilapia Fish pathogens |
| Note | A capstone project report submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Engineering Biosystems Engineering |
| Publisher | Asian Institute of Technology |
| Series Statement | Caps. Proj.; no. BSE-16-010 |
| Abstract | The aim of this study was to determine the antagonistic activity of probiotics isolated from Nile Tilapia (Oreochromis niloticus) against food-borne pathogens and fish pathogens. Fifty- four isolates were identified using 16S rRNA sequencing. Twenty Tilapia gut isolates and twelve LAB isolates from another study was used as Test strains. All the thirty-two strains were tested against four food-borne pathogens and four fish pathogens. Agar spot method was used to co-culture the test-indicator strains and the strains which were positive was then subjected to spot on lawn assay. All the test strains, except three Bacillus sp. exhibited an inhibition zones. In spot on lawn assay, non-neutralized culture supernatants with pH less than 4.0 showed inhibition zones against seven out of eight tested indicators. The neutralized culture supernatants of any of the test strains could not inhibit the indicators, except for the positive control. The culture supernatant of Lactococcus lactis was dialysed to obtain partially purified proteins, but concentrated products of the partial purification did not inhibit the growth of any indicators. |
| Year | 2016 |
| Corresponding Series Added Entry | Asian Institute of Technology. Caps. Proj. ; no. BSE-16-010 |
| Type | Capstone Project |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bachelor of Science in Engineering Biosystems Engineering (BSE) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Nipa Chokesajjawatee;Datta, Avishek;Loc Thai Nguyen; |
| Degree | Capstone Project (B.Sc.)-Asian Institute of Technology, 2016 |