Enzymatic hydrolysis of tilapia waste protein: effects of ultrasound treatment on hydrolysis and physicochemical properties of the hydrolysates | |
| Author | Arachchige, Dinushika P.S. Suraweera |
| Call Number | AIT Caps. Proj. no.BSE-18-10 |
| Subject(s) | Response surface methodology Alcalase Ultrasound Treatment Protein hydrolysate |
| Note | A capstone project report submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Engineering Biosystems Engineering |
| Publisher | Asian Institute of Technology |
| Series Statement | Caps. Proj. ; no. BSE-18-10 |
| Abstract | Industrial fish processing units generate massive amounts of wastes with high nutrient content. If these are not treated properly it may cause pollution and health problems. To overcome the challenges presented by the waste accumulated from the fish processing industry, waste is treated and used for various purposes. The byproducts acquired from fish processing can be a valuable source of proteins. 57.92% contains crude protein in fish waste. Hydrolysis can be done to unfold the three-dimensional structures of these proteins and for the easy accessibility of the proteins to produce value added products. Amongst acid, alkaline and enzymatic hydrolysis; enzymatic hydrolysis is better and eco friendlier. High pressure processing, ultrasound treatment, microwave irradiation is some of the methods used for the treatment of the hydrolysates. Most of the research conducted on the hydrolysis of pretreated proteins by one of the above methods. The primary objective of this study is to investigate the effect of ultrasound assisted enzymatic hydrolysis of tilapia waste protein. The effect of three independent variables; amplitude of ultrasound waves, time of ultrasound treatment and enzyme substrate ratio on degree of hydrolysis, soluble protein content, water holding capacity, oil holding capacity, solubility and emulsifying activity index was analyzed using Box Behnken design of response surface methodology. Emulsifying Activity Index model was significant according to the response surface methodology. Degree of hydrolysis, water holding capacity, emulsifying activity index at pH 7 and 10 increases with the higher amplitude. Solubility at pH 4, 7, 10, soluble protein content and emulsifying activity at pH 4 initially increases and then decreases with amplitude of ultrasound waves. Soluble protein content increase with time and solubility at all three pH. Time has little effect on water holding capacity, oil holding capacity, emulsifying activity. Degree of hydrolysis and solubility increase with enzyme substrate ratio. Future work can be done by using other proteolytic enzymes such as Papain to analyze the effect of type of enzyme on the ultrasound assisted enzymatic hydrolysis of tilapia fish waste protein. |
| Year | 2018 |
| Corresponding Series Added Entry | Asian Institute of Technology. Caps. Proj. ; no. BSE-18-10 |
| Type | Capstone Project |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bachelor of Science in Engineering Biosystems Engineering (BSE) |
| Chairperson(s) | Loc Thai Nguyen; |
| Examination Committee(s) | Anal, Anil Kumar;Salin, K. R.; |
| Degree | Capstone Project (B.Sc.)-Asian Institute of Technology, 2018 |