Extraction and characterization of astaxanthin from shrimp waste | |
| Author | Pathirannehelage, Saranya S.S.K. Yapa Yapa |
| Call Number | AIT Caps. Proj. no.BSE-18-15 |
| Subject(s) | Shrimp waste Astaxanthin Extraction Maceration Antioxidant activity |
| Note | A capstone project report submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Engineering Biosystems Engineering |
| Publisher | Asian Institute of Technology |
| Series Statement | Caps. Proj. ; no. BSE-18-15 |
| Abstract | Shrimp waste, which is produced in marine processing industries fetch a greater environmental risk as they are accumulating in larger quantities. These wastes are a major source of high valued natural carotenoids which can be utilized infeed, food, cosmetics and pharmaceutical industries. It is necessary to take proper initiatives to recycle these wastes in to valuable compounds. Amongst these carotenoids which were accumulated in the waste Astaxanthin is one of the major carotenoid which is capable in giving the red colour pigmentation for many marine crustaceans. Replacing synthetic Astaxanthin and high cost algal derived Astaxanthin from natural Astaxanthin derived from shrimp is environmentally as well as well as economically beneficial. This study was focused on assessing the extractability of Astaxanthin in different organic solvents (Acetone, Ethanol, Methanol, hexane, Petroleum Ether) of different polarities, in different extraction conditions including maceration and UAE and in different drying methods including oven drying, water bath evaporation, rotary evaporation and freeze drying. The extraction concentration of Astaxanthin obtained was compared within all the solvents, all the extraction methods and all the drying methods spectrophotometrically at 468nm and quantified using the equation obtained from drawing the standard curve for pure Astaxanthin. The highest concentration of Astaxanthin which is 50.03μg/g was given by using acetone as the extracting solvent, UAE as the extraction method and freeze drying as the drying technique. Then all the extracts obtained from different extraction methods and different drying methods were subjected to DPPH radical scavenging activity test in order to analyse the antioxidant properties and the inhibition (%) was calculated. The highest inhibition (%); 73.68%was recorded in the extracts obtained by using acetone as the extracting solvent, UAE as the extraction method and freeze drying as the drying technique. |
| Year | 2018 |
| Corresponding Series Added Entry | Asian Institute of Technology. Caps. Proj. ; no. BSE-18-15 |
| Type | Capstone Project |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bachelor of Science in Engineering Biosystems Engineering (BSE) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Bilal, Muhammad Sadiq;Loc Thai Nguyen; |
| Degree | Capstone Project (B.Sc.)-Asian Institute of Technology, 2018 |