Development and evaluation of a paper based biochemical sensor for realtime detection of food pathogen | |
| Author | Arachchillaya, Buddhika P. A. P |
| Call Number | AIT Caps. Proj. no.BSE-18-20 |
| Subject(s) | Paper based Biochemical Sensor Streptococcus agalactiae Tilapia fish |
| Note | A capstone project report submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Engineering Biosystems Engineering |
| Publisher | Asian Institute of Technology |
| Series Statement | Caps. Proj. ; no. BSE-18-20 |
| Abstract | Foodborne pathogens are a major public health threat and financial burden for the food industry, individuals, and society, as evidences say 76 million cases of food-related illness occurring in the United States alone each year. Rapid, sensitive, on-site detection of pathogens without a need for sophisticated equipment or skilled personnel is extremely important in health, industries and clinical scenarios. Here, the research work report a novel approach and new invention of paper based biochemical sensor to real time identify and evaluate a food pathogen. Biochemical sensor was made by a paper strip that contains cellulose microfibers with small pores, and entrapped with special chemical solution. To identification of pathogen, Streptococcus agalactiae which was taken as an indicator food pathogen. The whole conception of identifying a certain pathogenic bacteria through a paper based biochemical sensor was based on the matrix platform that use to fabricate paper sensor, substrates that use to break down through the bacterial metabolic activities and the pH indicator to indicate the desired pH value after the bacterial metabolic activities. So after the effect of pH change biochemical paper sensor was taken a unique color to indicate the target bacteria. The biochemical sensor was contained with two parts, sensor area, and hydrophobic area. So the identification of Streptococcus agalactiae, glycerol and peptone used as a substrate and after the breakage of substrate, the desired pH was indicated by phenol red. This was successfully used to detect Streptococcus agalactiae, when they are on tilapia fish with good response. |
| Year | 2018 |
| Corresponding Series Added Entry | Asian Institute of Technology. Caps. Proj. ; no. BSE-18-20 |
| Type | Capstone Project |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bachelor of Science in Engineering Biosystems Engineering (BSE) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Bilal, Muhammad Sadiq;Loc Thai Nguyen;Datta, Avishek; |
| Degree | Capstone Project (B.Sc.)-Asian Institute of Technology, 2018 |