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Use of hydrolytic enzymes to improve the nutrient utilization of cassava leaf meal | |
Author | Ekdanai Yodsuka |
Call Number | AIT Thesis no. AE-94-01 |
Subject(s) | Cassava |
Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development |
Publisher | Asian Institute of Technology |
Series Statement | Thesis ; no. AE-94-01 |
Abstract | Cassava ( Manihot esculenta) leaf was treated with enzymes namely, cellulase, pectinase, papain and bromelain. Only cellulase was succeed in cassava leaves treatment in term of degradation cellulose in cassava leaves to glucose by Glucostat as the method of glucose determination . Other enzymes such pectinase, papain and bromelain showed no reaction with cassava leaves as substrate. The optimum conditions of cellulase enzyme are pH 4.8 and temperature 50°C . The optimum of cassava leaves concentration for cellulase reaction is 25.0% (w/v) and the optimum cellulase concentration is 1 . 0% (w/w). The reaction time was indicated when the reaction was taken placed in 10 liter reactor which pointed 6 hours for the reaction time. Treated cassava leaves was recovered by oven dry at 70"C for 24 hours which provided 10.86% in fiber content (lower than untreated sundried cassava leaves) and higher in ash content i.e . 12.00%. Daily Weight Gain (DWG) of fish fed by treated cassava leaves (cellulase treatment) was 0 . 68 which was not significant higher than untreated cassavs leaves . For feed utilization of fish fed by treated cassava leaves there were 59.53% in Apparent Prate in Digestibility ( APD) , 5 8. 60% in Total Digestibility (TD), 15.26% in Apparent Net Pratein Utilization ( ANPU) and 1. 0% . in Protein Eff iceincy Ratio (PER) which were comparable to untrated cassava leaves. |
Year | 1994 |
Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AE-94-01 |
Type | Thesis |
School | School of Environment, Resources, and Development (SERD) |
Department | Other Field of Studies (No Department) |
Academic Program/FoS | Agricultural and Food Engineering (AE) |
Chairperson(s) | Yakupitiyage, Amararatne |
Examination Committee(s) | Edwards, Peter;Sarath Ilangantileke;Little, David |
Scholarship Donor(s) | International Development Research Center (IDRC, Canada) |
Degree | Thesis (M. Eng.) - Asian Institute of Technology, 1994 |