Effect of carbon sources and pretreatment with probiotics on ammonia reduction in aquaculture water | |
| Author | Virgula, Juliette Caumban |
| Call Number | AIT Thesis no.AQ-20-03 |
| Subject(s) | Ammonia--Toxicology Probiotics Aquaculture--Biotechnology |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management |
| Publisher | Asian Institute of Technology |
| Abstract | Biofloc technology is used to control ammonia in aquaculture water and this is generally achievable by adding a carbon source, however, the capacity of biofloc in controlling ammonia might be influenced by the type of carbon source used. This study evaluated the effectiveness of simple and complex carbohydrates (glucose and tapioca starch) as carbon sources in the reduction of ammonia content in the water. The effect of pretreating the carbon source by simply adding Bacillus spp. and by fermentation of starch with Bacillus spp. were also evaluated. The experiment consisted of a control (no addition of carbon source) treatment, carbon source alone (glucose only, GO and starch only, SO) treatments, combination of probiotic bacteria Bacillus spp. with both carbon sources (glucose + bacteria, GB and starch + bacteria, SB) treatments and starch fermented for 24 h (FS). The water quality parameters were monitored for 72 h after the addition of the different carbon sources. All the carbon source treatments were effective in reducing the total ammonia nitrogen (TAN) concentration in the water column for 48 h compared to control. GB and FS significantly reduced (p < 0.05) 20% of TAN level in the water within 6 h after application. The increase or decrease of added probiotics and reduction of starch content during fermentation were evaluated by total plate count of bacteria and estimation of the remaining starch content after 24-h fermentation of tapioca starch with Bacillus spp. The results showed a significant increase (7.13x103%) in bacteria counts in the fermented solution. Fermentation, however, did not completely hydrolyze starch into simple sugars as tapioca starch was partially soluble in the water. The reduction in starch content after fermentation was only 3%. |
| Year | 2020 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Salin, Krishna R. |
| Examination Committee(s) | Yakupitiyage, Amararatne;Bhujel, Ram C.;Visvanathan, Chettiyappan |
| Scholarship Donor(s) | Thai Pipe Scholarship;Asian Institute of Technology Fellowship |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2020 |