1 AIT Asian Institute of Technology

Removal of antinutrients from bitter cassava leaves and its effect on feed quality for Nile tilapia

AuthorShukla, Arvind Kumar
Call NumberAIT Thesis no. AE-92-30
Subject(s)Fishes--Feeding and feeds

NoteA thesis submitted in partial fulfilment of the requirements for the degree of Master of Science, School of Environment, Resources and Development
PublisherAsian Institute of Technology
AbstractBitter cassava (Manihot esculenta) leaf was investigated for the presence of antinutrients namely, trypsin inhibitor (TI), cyanogen, phytate, tannin and saponin. Treatments namely, boiling (Tl), soaking (T2). alkali treatment (T3), drying (T4) and ensiling (T5) were evaluated for their efficacy to remove these antinutrients. On the basis of chemical analysis the treatments were selected and differently treated cassava leaf meal (CLM) based diets were prepared. These diets were evaluated for their suitability in fish grow-out experiment. The amount of Tl, HCN and phytic acid in untreated leaf were found to be 377 .6, 42.0 and 803.3 mg/kg, respectively. Tannin and saponin contents were found to be 2.17 and 2.53%, respectively. Efficiency of the soaking and alkali treatment for removal of most of the antinutrients was generally high. To remove TI and HCN, alkali treatment, drying and ensiling, however, were found to be equally good. Saponin and phytic acid were best removed by boiling while tannin removal was most efficient by soaking followed by alkali treatment. Ensiling resulted in increased tannin content. The treatments selected for the grow- out experiment were drying, soaking, alkali treatment, and boiling. The treated dry leaf was powdered and isonitrogenous diets were prepared and fed to Nile tilapia for 42 days. None of the treatments resulted iii a reduction of crude protein content of CLM but the ash content was significantly reduced after every treatment except drying. In comparison to control diet (Dl) fed fish the growth rate (GR) and specific growth rate (SGR) of fish was very low when fed CLM based diets. However, soaked and alkali treated CLM based diets showed comparatively better results (0.44 and 0.40 g/day GR,respectively). The total digestibility (TD) and apparent protein digestibility (APD) of CLM based diets were approximately 20 and 55%, respectively, while apparent net protein utilisation (ANPU) of these diets were around 15%. The protein efficiency ratio (PER) of these diets were around 0. 7. The food conversion ratio (FCR) of boiled CLM based diet was highest and that of control diet was lowest. All the digestibility and feed utilisation parameters showed that the CLM based diets were poorer as compared to control diet, however, soaked and alkali treated CLM based diets were comparatively better. There was a significant loss of carcass lipid of fish fed CLM based diets showing energy limitation of these diets. On the basis of the fish grow- out experiment it was concluded that soaking and alkali treatments were the best to remove most of the antinutrients efficiently for incorporation of CLM in fish diet. However, the low dry matter digestibility of treated cassava leaf meal remained as a major constraint.
Year1992
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSAgricultural and Food Engineering (AE)
Chairperson(s)Yakupitiyage, Amararatne
Examination Committee(s)Edwards, Peter ;Little, David C.
Scholarship Donor(s)The Royal Netherlands Government;
DegreeThesis (M.Sc.) - Asian Institute of Technology, 1992


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