1 AIT Asian Institute of Technology

Investigation into the immersion of known-age Oreochromis niloticus juveniles for hormonal sex reversal

AuthorYang, Yi
Call NumberAIT Thesis no. AE-92-32
Subject(s)Fishes--Sexing

NoteA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development
PublisherAsian Institute of Technology
AbstractThe present study dealt with the determination of the effect of juvenile immersion in solutions of 17a- methyltestosterone (MT) for different durations and at different ages, and the determination of the critical period of sexual differentiation of Oreochromis niloticus by inference. The results indicated that fish growth in terms of mean individual fish weight and specific growth rates were not significantly different between all treatments during Phase I (p > 0.05) and were positively correlated to male percentages of treatments (p < 0.001 , r = 0.68, n = 20 and p < 0.05, r = 0.73, n = 20, respectively) during Phase II, and food conversion ratios were highly positively and negatively correlated to male percentages of male during Phase I (p < 0 . 001, r = 0.71, n = 20) and Phase II (p < 0.05, r = - 0.48, n = 20), respectively. Fish survival rates did not significantly vary amongst treatments (p > 0.05) and were not significantly correlated to male percentages (p > 0 .05 ) during the period of 17u-methyltestosterone treatment and in subsequent culture. Control-I (MT/DMSO-free ), control-II (MT-free) and three MT-treatments starting from the 6th day to the 15th day and 30th day after hatching and starting from the 15th day to the 18th day after hatching had sex ratios that did not differ significant l y from the expected 50:50 mal e to female ratio (p > 0.05, Ch i -square ). Sex ratios of a ll other treatments including the treatment fed MT-feed were significantly different from the expected 50:50 male to femael ratio (p < 0.0 5, Chi- square). The treatments with highest male percentages were 24-30 starting from the 24t h day to the 30th day ( 893), 21-24 (893), 21-30 (903), 24-27 (923), 21-27 (943 ) and 27-30 (943 ). This indicated that the starting time of administration of 17umethyltestosterone had crucial effect on the success of sex reversal. It can be concluded that the most labile period of sexual differentiation of Oreochromis niloticus appeared to be between the 21st day and the 30th day after hatching . The present study indicated that the treatments starting during the most labile period of sexual differentiation was much more effective than those starting from the sexually undifferentiated stage and continuing through the stages of gonadal sexual differentiation which was suggested by Yamamoto (1969) and followed by almost all authors.
Year1992
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSAgricultural and Food Engineering (AE)
Chairperson(s)Little, David C.;
Examination Committee(s)Yakupitiyage, Amararatne ;Phillips, Michael J.
Scholarship Donor(s)The Government of Federal Republic of Germany (DAAD);
DegreeThesis (M.Sc.) - Asian Institute of Technology, 1992


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