1 AIT Asian Institute of Technology

Pathogen indicator microorganisms for waste stabilization pond system

AuthorNishimura, Sosuke
Call NumberAIT Thesis no.EV-92-03
Subject(s)Sewage lagoons
NoteA thesis submitted in partial fulfillment of the requirement for the degree of Master of Engineering NOTE
PublisherAsian Institute of Technology
AbstractWater samples collected from two stabilization pond systems (AIT ponds in Patumthani province , and Rama IX ponds in Bangkok city , Thailand) were analyzed to correlate the densities of indicator microorganisms , namely , fecal coliform and coliphage, to the presence of pathogenic bacteria such as ViJJrio clwlerae, Salmonella , and Shigella. Out of 103 pond water samples, 84 samples were found to contain v. clwlerae. Statistical analysis showed that both fecal coliform and coli phage had good correlations with the presence of V. clwlerae though coliphage was less reliable. The proportions of V. clwl erae- posi ti ve samples were significantly h igher in the high fecal coliform sample group ( > 8000 fecal coliforms per 100 mL) than in the low fecal coliform sample group ( 5 8000 fecal coliforms per 100 mL). As for coliphage, the threshold level was found to be 1300 coliphages per 100 mL. The percentage of V. clwl erae positive sample s (percent VC - pos itive) in the total number of samples was calculated for each sampling point to analyze quantitative relationships between the V. chol erae presence and the indicator densities. Two 1 inear regression formulas were developed to estimate the percent VCpositive based on fecal coliform or col iphage d ensity. Applying the concept of the most probable number (MPN) t e st, a formula to estimate the MPN of V. cholerae based on the percent VC - posi tive was s ugge sted . Using the thre e formulas mentione d a bo ve , approx ima t e c once ntration of V.cholerae in pond water could be estimate d from fe c al c oliform or coliphage density.
Year1992
Corresponding Series Added EntryAsian Institute of Technology.|tThesis ;|vno.EV-92-03
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC))
Academic Program/FoSEnvironmental Engineering and Management (EV)
Chairperson(s)Chongrak Polprasert
Examination Committee(s)Samorn Muttamara;Tran, Francis T.
Scholarship Donor(s)The Government of Japan
DegreeThesis (M.Eng.) - Asian Institute of Technology, 1992


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