Saccharification of cassava starch assisted by an ultrafiltration reactor | |
| Author | Huss, Martine |
| Call Number | AIT Thesis no.AE-91-22 |
| Subject(s) | Ultrafiltration Starch |
| Note | A thesis manuscript submitted in partial fulfillment of the requirements for the degree of Master of Science |
| Publisher | Asian Institute of Technology |
| Abstract | Conventional batch processes for cassava starch hydrolysis, i.e, acid, acid/enzyme and enzyme/enzyme hydrolysis, were compared. The effect of enzyme concentration on liquefaction and saccharification was also investigated for acid/enzyme and enzyme/enzyme hydrolysis. Results showed that the enzyme/enzyme process with a-amylase at 0.3% and glucoamylase at 0.7% was the most efficient. Enzyme/enzyme hydrolysis in an UF reactor was performed in order to optimize the hydrolysis process by recycling the enzyme. Results were analyzed in terms of quantity and quality of the out-coming glucose syrup, i.e, total reducing sugars, total solids, maltose and glucose. Substrate concentration, and operating parameters such as flow rate and pressure were tested. The results showed that 10% w/w of starch was the optimum concentration for the UF reactor. The transmembrane pressures tested, which ranged from 30 to 50 psi, showed no significant effect on the flux rate. A flow rate of 400 1/h improved the flux rate and thus the passage of sugars through the membrane, compared to 300 1/h. A continuous starch hydrolysis by operating the UF reactor over 8 hours could be performed. A continuous increase in reducing sugar could be obtained compared to the conventional enzyme/enzyme batch process. |
| Year | 1991 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Agricultural and Food Engineering (AE) |
| Chairperson(s) | Athapol Noomhorm; |
| Examination Committee(s) | Ilangantileke, S.G.;Jindal, Vinod Kumar; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1991 |