1 AIT Asian Institute of Technology

An investigation into the gonadal maturation and development of spawning methods and breeding practices of the silver barb (Puntius gonionotus)

AuthorDjumanto
Call NumberAIT Thesis no. AE-93-23
Subject(s)Fishes--Spawning, Induced
Fishes--Breeding
NoteA Thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development
PublisherAsian Institute of Technology
AbstractThe use of gonadotropin releasing hormone analogue (LH—RH-A) combined with dompeTidone , a dopamine antagonist , to induce ovulation and spawning in silver barb (Puntius gonionotus) has been demonstrated both at the beginning and middle of the season. Dose levels 5 ug, 10 ug and 15 pg/kg body weight were compared. There was no relationship between dose and most indicators of spawning performance, except time latency which demons t rated that in the higher dose fish spawned faster , A higher dose in the early season gave lowest variability, in fecundity however, A circular flow was created using 3 sizes of submersible pumps, namely BIO L/minutes, x 15 L/minutes and L/ minutes. Increase velocity depressed spawning performance in terms of relative fecundity. The action common carp (Cyprinus carpio) spawning in the same tanks as silver barb could stimulated the latter to ovulate and spawn Spawning rate was low but release of eggs after manual stripping, indicated that a ratio 3 1 (numbers of common carp silver barb) stimulated ovulation in 50 % of female fish. The result of respawning fish after different duration since their last ovulation demonstrated that this fish is able to spawn every month without affecting spawning performance in terms of spawning success, fecundity, fertility , hatching rate. Fish could spawn after only 15 days but fecundity was reduced, Microscopic investigation of the ovaries before and after spawning showed that the ovaries of the silver barb contained oocytes of different stages. During spawning the fish expel led al l the mature oocytes in the dormant phase (more than 600 11m in diameter) leaving primary oocytes. ACKNOWLEDGEMENTS The author wishes to express his sincerest and profound gratitude to his advisor, Dr, David Little for his encouragement, and guidance throughout the period of study. The author also whises to express his sincere thanks to Dr. Amararatne Yakupitiyage and to Prof, Peter Edwars for their encouragement , advise , constructive criticism and valuable guidance as members of the committee . Special thanks are given to the Aquaculture laboratory staff and workers for providing assistance during conduct the study. Sincere appreciation is due to al l good friends, especially Mr. Setiato, Mr. Karim, Mr. Tri Djoko for their assistance during the study, as well as to Mr. Haryono for his encouragement , The author wishes to acknowledge the Asian Institute of Technology for providing a commendable learning experience and also to his donor, The Government of Nether lands, for providing the scholarship through A IT . Deep love and gratitude is given to his wive, SUKMA RIDARWATI , for her steadfast love, prayers and moral support that inspired the author to pursue his studies . This piece of work is therefore dedicated to her for al l sacrificing and understanding that has been given to him which lead to finished his study in A IT
Year1993
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentOther Field of Studies (No Department)
Academic Program/FoSAgricultural and Food Engineering (AE)
Chairperson(s)Little, David C.
Examination Committee(s)Yakupitiyage, Amararatne ;Edwards, Peter
Scholarship Donor(s)Netherlands
DegreeThesis (M.Sc.) - Asian Institute of Technology, 1993


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