Design and fabrication of aptamer-based SERS chips for selective detection of paraquat herbicide residues in water and soil | |
| Author | Napatsakorn Kamkrua |
| Call Number | AIT Thesis no.ISE-22-10 |
| Subject(s) | Raman spectroscopy Biosensors |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Nanotechnology |
| Publisher | Asian Institute of Technology |
| Abstract | A common herbicide such as paraquat has been countlessly applied in agriculture, and its residues are left in water and soil. Paraquat toxicity highly damages the body, which can be lethal in a later stage. Thus, paraquat detection is important and several techniques have been developed in recent years. Raman spectroscopy facilitates herbicide detection in rapid and non-destructive ways. Furthermore, surface-enhanced Raman spectroscopy (SERS) and bioreceptors are commonly utilized to enhance the sensitivity and selectivity of molecular structure detection, respectively. This study focuses on optimizing SERS-active substrates to improve the selectivity and rapidity of paraquat detection. This work also aimed to develop the combinations of SERS and biosensors for paraquat detection by functionalizing metallic nanostructures with an aptamer that is specific to the paraquat molecule via Raman spectroscopy. Soil and water samples were used for the evaluation of optimized SERS-active substrates. The sensitivity, selectivity, and limit of detection were investigated by Raman measurement. This study optimized the ONSPEC-Prime SERS chip (AgNRs) for paraquat detection in water and soil samples. Without extraction protocol, the chip provided a sensitivity of 638.96 a.u./µM and a detection limit (LOD) of 0.1 µM for the water sample. However, in the soil samples, paraquat extraction protocol with alumina absorbent is required. Furthermore, the aptamer-based SERS chip was successfully fabricated with a sensitivity of 609.15 a.u./µM and LOD of 0.35 µM using 0.1 µM of aptamer immobilized on the ONSPEC-Lite SERS chip (AuNPs). |
| Year | 2022 |
| Type | Thesis |
| School | School of Engineering and Technology (SET) |
| Department | Department of Industrial Systems Engineering (DISE) |
| Academic Program/FoS | Industrial Systems Engineering (ISE) |
| Chairperson(s) | Bora, Tanujjal;Noppadon Nuntawong (Co-Chairperson) |
| Examination Committee(s) | Ricco, Raffaele;Loc Thai Nguyen |
| Scholarship Donor(s) | Royal Thai Government Scholarships |
| Degree | Thesis (M. Eng.) - Asian Institute of Technology, 2022 |