Production of citric acid from pineapple waste via fermentation | |
| Author | Sun, Guanwen |
| Call Number | AIT Thesis no. EV-84-16 |
| Subject(s) | Citric acid Agricultural wastes--Environmental aspects |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | Raw liquid obtained from juice of the pineapple solid waste which was discarded by the cannery was used as a fermentation medium for the production of citric acid. The chemical analysis showed t hat the raw liquid contained about 5-7 % sugar which increased to 7-8 after autoclaving. The study proved the suitability of raw liquid for fermentation. Inoculum identified under the microscope was named Aspergillus niger MAT- 1 which is a wild type strain from tropical countries. Shake flask studies at 30 c on a rotary shaker of 150 rpm indicated that the optimum conditions for fermentation are as follows: inoculum size, 10 % (V/V) spore suspension. of 50 % transmittance ; fermentation duration, 144 hours; pH, 3.0 -4.0 ; added phosphate (KH2 .PO4) , 0.5-1.0 g/liter. The yields of the citric acid varied from 5.0-13.6 g/liter of the liquid , depending on sugar content in the samples. Addition of nitrogen compounds not only markedly reduced the amount of citric acid formed , but also reduced fungus growth. The addition of methanol at a concentration of 2-4 % proportionally increased the formation of citric acid from raw liquid of the waste. However , the yield of citric acid was suddenly dropped at methanol. concentration of 5%. On the other hand, the mycelia l growth was markedly increased with addition of methanol at the first, then slowly reduced. Pilot plant studies in a fermenter indicated that yield of citric acid was 5.65 g/liter of fermentation liquid, and 36.8 % on the basis of the sugar consumed. Yield of mycelial dry weight was 14.6 g/1 and 65.5% based on the sugar consumed. It contains 27.3 % protein and is a good source of single-cell protein |
| Year | 1984 |
| Type | Thesis |
| School | School of Environment, Resources, and Development |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Samorn Muttamara ;Nguyen, Cong Thanh; |
| Examination Committee(s) | Vigneswaran, Saravanamuthu ; |
| Scholarship Donor(s) | Carl Duisberg-Gesellschaft e. v ., The Federal Republics of Germany ; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1984 |