In situ monitoring of enzymatic hydrolysis of tapioca starch : electrochemical measurement and kinetics modelling | |
| Author | Emurigho, Tega Anthony |
| Call Number | AIT Thesis no.FB-24-01 |
| Subject(s) | Starch Tapioca Electrochemistry |
| Note | A Thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology |
| Publisher | Asian Institute of Technology |
| Abstract | Non enzymatic screen printed carbon electrodes have found their application in the monitoring of glucose in urine and blood samples due to its low cost, sensitivity and selectivity. However, its application in this study was in real time monitoring of glucose released from the hydrolysis of native, acetylated modified and thermally treated tapioca starch using alpha amylase subjected to temperatures of 400C and 500C, pH 5.0 and 7.0, and reaction time of 0 - 60 min with the kinetics modelling of the digestogram. The results of the kinetics of the electrooxidation of glucose at the temperature of 40oC, pH 5.0 and 60 min showed the thermally treated tapioca starch with glucose concentration of 4.87 mM, native tapioca starch, 3.40 mM and acetylated modified tapioca starch 0.93 mM respectively. Also, the kinetics modelling of the digestogram revealed that both the first order kinetic and Chapman Richard’s function models had good predictions of the hydrolysis process, however, the Chapman model had a better prediction than the first order kinetic model. |
| Year | 2024 |
| Type | Thesis |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Loc, Thai Nguyen; |
| Examination Committee(s) | Anal, Anil Kumar;Salin, Krishna R.; |
| Scholarship Donor(s) | Tertiary Education Trust Fund (TETFund), Nigeria; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2024 |