Effect of NA-CID and beta sac plus dietary additives on growth performance and health of pacific white shrimp (Litopenaeus vannamei) | |
| Author | Devendra, Dhuri Nimish |
| Call Number | AIT Thesis no.AQ-22-02 |
| Subject(s) | Shrimps--Growth Whiteleg shrimp Shrimp culture |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management |
| Publisher | Asian Institute of Technology |
| Abstract | This study examined the effects of adding two commercial products, viz., Na-Cid and Beta Sac Plus, on the growth performance and health of Pacific white shrimp (Litopenaeus vannamei). The Na-Cid powder contained a mixture of pineapple (98%) and turmeric (2%) extracts, while the Beta Sac Plus powder was a combination of fermented yeast (96%) with inulin (4%). There were four treatments with four replicates in this trial: (1) T1 (no feed additive), (2) T2 (adding 20 g/kg Na-Cid powder to shrimp feed), (3) T3 (adding 20 g/kg Beta Sac Plus Powder) and (4) T4 (adding 20 g/kg Na-Cid powder + 20 g/kg Beta Sac Plus Powder). A total of 1,600 shrimps with an initial body weight of 2.50 ± 0.03 g were randomly distributed into 16 circular tanks. The feeding trial was conducted in a greenhouse using a recirculating aquaculture system (RAS). All shrimps were hand-fed the experimental diets four times daily (7.00, 11.00, 15.00 and 19.00 h) with at 5% of body weight for 56 days. The results showed that the survival was significantly increased in the single and combined supplementation of both feed additives (T2, T3 and T4 groups) (P<0.05). There were no significant differences (P>0.05) in final body weight, weight gain (WG), specific growth rate (SGR), feed conversion ratio (FCR) and condition factor (CF) among all groups. The shrimp hepatopancreas weight was significantly increased in T4 group (P<0.05). There was no significant difference in the shrimp’s whole body composition (P>0.05). There was no significant difference in the intestinal protease enzyme activity between T1 and T4 groups (P>0.05), but the T1 group had a significantly greater protease activity in hepatopancreas compared to other groups (P<0.05). The number of R-cells in the hepatopancreas was significantly higher in T4 group (P<0.05). The total count of Vibrio spp. in hepatopancreas was significantly decreased in all feed additives supplemented groups (P<0.05). The cumulative mortality of shrimp was significantly lower (P<0.05) in T2 under chronic exposure to ammonia toxicity. The T4 group showed a higher gross profit margin (%) than other groups as revealed by the economic feasibility analysis. The results of feed utilization parameters such as protein intake (PI), lipid intake (LI), protein efficiency ratio (PER), lipid efficiency ratio (LER), apparent net protein utilization (ANPU), and apparent net lipid utilization (ANLU)) were inconclusive because of the limitations in the nutrient composition analysis of experimental diets. In conclusion, the single and combined supplementation of Na Cid powder and Beta Sac Plus powder could not improve the growth performance of shrimp. Still, both feed additives could promote shrimp survival in the feeding trial. However, the dietary supplementation of Na Cid powder at 20 g/kg feed could reduce shrimp mortality associated with stress under chronic ammonia exposure. |
| Year | 2022 |
| Type | Thesis |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Salin, Krishna R.;Ha, Thanh Dong (Co-Chairperson); |
| Examination Committee(s) | Yakupitiyage, Amararatne;Zulfiqar, Farad;Lumpan Poolsawat,; |
| Scholarship Donor(s) | AIT Scholarships; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2022 |