Impact of ozone nanobubble application on immunity and disease resistance in post-larval pacific white shrimp (Penaeus vannamei) against vibrio parahaemolyticus | |
| Author | Sarawut Thongpocha |
| Call Number | AIT Thesis no.AQ-24-01 |
| Subject(s) | Shrimps--Diseases Natural immunity Vibrio parahaemolyticus |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management |
| Publisher | Asian Institute of Technology |
| Abstract | Pacific white shrimp farming plays a significant role in the industry. However, farmers are encountering challenges associated with Vibrio parahaemolyticus, a disease that remains resilient. Ozone nanobubbles show that it has been implemented to enhance immunity and combat bacterial infections, and influent fish immunity. However, there is no information regarding the effect of ozone nanobubbles on the innate immunity of shrimp. This study focused on identifying the safety level of ozone nanobubbles on post larval shrimp. Subsequently, an examination of innate immune genes expression post treatment was conducted, followed by a simulation of transport under controlled laboratory conditions. Then, we explore disease resistance of ozone nanobubble treated post-larval shrimp against V. parahaemolyticus. The results showed that one minute ozone nanobubbles resulting in no mortality and ensuring the safety of shrimp in the post-larval stage. Furthermore, shrimp that received ozone nanobubble treatment displayed enhanced expression of Lysozyme, ProPO2, cmSOD, and ALF immune genes. Besides, there was a noticeable up-regulated in the levels of immune genes expression during the 3-hour transportation period. Similarly, after being infected with Vibrio parahaemolyticus, the shrimp displayed the highest survival rate post-infection. In summary, ozone nanobubble treatment might enhanced shrimp immunity and survival when encounter virulent pathogens, beneficial for maintaining optimal dissolved oxygen levels without harming post-larval shrimp. |
| Year | 2024 |
| Type | Thesis |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Salin, Krishna R.; |
| Examination Committee(s) | Ha, Thanh Dong;Loc, Thai Nguyen; |
| Scholarship Donor(s) | Royal Thai Government Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2024 |